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Inhibition of excision of three types of DNA lesions by nucleosomes. The indicated substrates, either as naked DNA or in nucleosomes (Nuc), were incubated with 6-factor reconstituted excision nuclease for 3 h at 30°C, and the products were separated on an 8% denaturing polyacrylamide gel. The figure shows an autoradiogram of the gel. The levels of excision were quantified by PhosphorImager and the ImageQuant system and are indicated at the bottoms of all lanes.
