FIG. 6.

TLP is translocated to the nucleus when cells are exposed to stress agents. (A) UV-irradiated DT40 cells (100 J/m²) were harvested at the indicated times after irradiation, and TLP and β-actin in the nuclear and cytoplasmic extracts were analyzed by Western blotting. NIH 3T3 (B) and HeLa (C) cells were exposed to UV light (100 J/m²), and the nuclear extracts were analyzed as described for panel A. Cytoplasmic and nuclear extracts were applied to 1/10 and 3/4 of the total extracts from the same number of cells (approximately 10⁶), respectively. (D) NIH 3T3 cells exposed (panel 3) or not exposed (panels 1 and 2) to UV light (100 J/m²) were harvested 15 min after irradiation, and the localization of TLP (panels 2 and 3) and α-tubulin (panel 1) was analyzed immunocytochemically.