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. 2003 Jun;23(12):4307–4318. doi: 10.1128/MCB.23.12.4307-4318.2003

FIG. 8.

FIG. 8.

Reduced DNA replication and transcription in TAF10−/− trophoblasts. (A) DNA synthesis in trophoblast cells. After 16 h of culture in the presence of BrdU, the BrdU incorporation in TAF10−/− (−/−) and wild-type (wt) trophoblasts was visualized by anti-BrdU immunofluorescence and counterstained with DAPI. Magnification, ×630. (B) In situ run-on transcription in trophoblast cells. Blastocyst outgrowths grown on coverslips for 2 days were subjected to in situ run-on assays essentially as described elsewhere (43). Br-UTP incorporation in nascent RNAs in Triton X-100 permeabilized TAF10−/− or wild-type (wt) trophoblasts visualized with an anti-BrdU antibody counterstained with DAPI. Magnification, ×630.