FIG. 3.

Nuclear colocalization of MLL-ELL with p53 requires ELL(eCT) and p53(TAD). 293T (A and B) or U2OS (C and D) cells were cotransfected with 4 μg of the indicated constructs and after 24 h were stained with anti-ELL antibody as described in Materials and Methods. Cells were visualized by using a fluorescence microscope, and representative fields were photographed. The images show GFP-p53 (green); MLL-ELL, ELL, or their truncated mutants (red); and nuclei (DAPI; blue). Merge images were produced by using SPOT advanced software. An enlarged view of representative cells coexpressing various constructs is also shown.