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. 2003 Jun;23(12):4230–4246. doi: 10.1128/MCB.23.12.4230-4246.2003

FIG. 6.

FIG. 6.

ELL(eCT) is necessary and sufficient to suppress both p53- and p53(6KR)-mediated induction of p21. (A) H1299 cells were transfected with the following constructs using Lipofectamine: Flag-p53 (1 μg) or MLL-ELL or MLL-ELLΔCT (5 or 10 μg of each cDNA). pEGFP (0.5 μg) was included as a transfection efficiency control. (B) A similar experiment was conducted with 2 and 5 μg of Flag-ELL, Flag-ELLΔeCT, and Myc-ELL(eCT). Cell lysates prepared 24 h posttransfection were probed with the indicated antibody. (C) H1299 cells transfected with Flag-p53 (0.3 μg), Flag-p53(6KR) (0.3 μg), and HA-p300 (1 μg) were harvested 24 h posttransfection, and cell lysates were analyzed by Western blot with the indicated antibody. (D) H1299 cells were transfected as shown with p53(6KR) (1 μg) and ELL constructs (2 and 5 μg), and after 24 h the cell lysates were analyzed with the indicated antibody.