FIG. 7.

ELL(eCT) is necessary and sufficient to inhibit p53-induced apoptosis. (A) Flag-p53 (1 μg), MLL-ELL or MLL-ELLΔCT (5 and 10 μg each), and Us9-GFP (0.5 μg) were transfected into H1299 cells with Lipofectamine. After 36 h of incubation, cells were fixed in 70% ethanol for 24 to 72 h and stained with propidium iodide solution. Approximately 10,000 GFP-positive cells from each sample were then analyzed for their DNA content by using flow cytometry. The percentage of cells in sub-G₁ phase is shown as the mean ± the SD of three independent experiments. Representative histograms from each transfection are also shown. (B) An experiment identical to that in panel A was conducted with Flag-ELL, Flag-ELLΔeCT, and Myc-ELL(eCT) (5 and 10 μg each).