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Xkid is degraded in a D-box-independent pathway. CSF (CSF+Ca2+) and interphase extracts (INT+Cdh1 and INT-Cdh1) were treated as in Fig. 2D except for the addition of either a radiolabeled wild-type Xkid (X-kid Wt) or a Xkid protein in which the five D-box sequences were point mutated [ΔD-box(1-5)]. The amount of Xkid remaining with time is quantified from the autoradiographs.
