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Analysis of DNase I hypersensitivity. HT-1080 (male human fibrosarcoma) cells and Δ3B (HT-1080 cells carrying the promoter mutation) cells were treated with 0, 10, 20, 30, 40, 50, 60, 70, 80, 90, and 100 μg of DNase I/ml. Southern blot analysis was performed on DNA digested with EcoRI and was hybridized to the HPRT promoter probe HPRT A. “HS site” indicates the positions of HPRT-hypersensitive sites; “globin” indicates the hybridization band detected by the control human β-globin probe.
