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. 2003 Jun;23(12):4267–4282. doi: 10.1128/MCB.23.12.4267-4282.2003

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Copyright © 2003, American Society for Microbiology

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FIG. 8. — Effect of MAGI-1 on the cell adhesive activity of JAM4. (A) Phase-contrast images of L-FLAG-JAM4 (a), L-FLAG-JAM4ΔC (b), L-FLAG-JAM4/Myc-MAGI-1 (c), and L-FLAG-JAM4ΔC/Myc-MAGI-1 (d) cells grown in cultures on plastic dishes. Bar, 50 μm. (B) Immunofluorescence of FLAG-JAM4 in L-FLAG-JAM4 (a) and L-FLAG-JAM4/Myc-MAGI-1 (b) cells. Bar, 10 μm. (C) Phase-contrast images of L-FLAG-JAM4 (a) and L-FLAG-JAM4/Myc-MAGI-1 (b) cells in collagen cultures. Bar, 100 μm. (D) Dissociation assay of L-FLAG-JAM4 and L-FLAG-JAM4/Myc-MAGI-1 cells. (a) L-FLAG-JAM4 cells after being pipetted five times; (b) L-FLAG-JAM4/Myc-MAGI-1 cells after being pipetted five times. Bar, 200 μm. (c) Western blot with the anti-FLAG antibody of L-FLAG-JAM4 and L-FLAG-JAM4/Myc-MAGI-1 cells. Lane 1, L-FLAG-JAM4 cells; lane 2, L-FLAG-JAM4/Myc-MAGI-1 cells. The mobilities of molecular mass standards (in kilodaltons) are indicated at the left.