FIG. 3.

A deacetylase activity is required for cytokine induction of STAT5 target genes. (A) Three unrelated inhibitors of deacetylases block induction of STAT5 target genes. Ba/F3-β cells were stimulated with IL-3 in the presence of 200 nM TSA, 10 μM SAHA, 10 mM NaB, or DMSO (−), and mRNA levels were analyzed by real-time PCR, as described for Fig. 2. (B) Titration of TSA and SAHA. Ba/F3-β cells were stimulated with IL-3 for 30 min in the presence of increasing concentrations of TSA or SAHA, and mRNA levels were analyzed by real-time PCR, as described above. Data are expressed as the fold induction relative to untreated unstimulated cells.