FIG. 6.

TSA does not affect chromatin remodeling on the Cis promoter. (A) Structure of the Cis promoter, indicating the positions of the restriction sites analyzed for panel B. Gray boxes represent STAT5 binding sites. (B) IL-3-mediated chromatin remodeling on the Cis promoter is not affected by TSA. Ba/F3-β cells were stimulated with IL-3 for 30 min in the absence or presence of 200 nM TSA, as described for Fig. 1. Unstimulated and stimulated cells were harvested, nuclei were prepared, and chromatin accessibility was analyzed by CHART-PCR, as described in Materials and Methods. Purified genomic (naked) DNA was included in a parallel reaction as a control for the cutting efficiency of the restriction enzyme. Data are expressed as the percentages of nondigested DNA (% Protection).