TABLE 1.
DNA microarray analysis of gene expression in IL-3-stimulated Ba/F3-β cells in response to TSA
| Protein or gene (gene designation)a | Accession no. | Relative microarray expression (fold) after 0.5 and 2 h of IL-3 stimulationc
|
STAT5 target genesd | Group | |||
|---|---|---|---|---|---|---|---|
| −TSA
|
+TSA
|
||||||
| 0.5 h | 2 h | 0.5 h | 2 h | ||||
| Downregulated by TSA | |||||||
| Cytokine-inducible SH2-containing protein (Cis)b | D31943 | 10.1 | 8 | 3 | 2.3 | + | A |
| Myelocytomastosis oncogene (c-Myc)b | X01023 | 8.4 | 5.5 | 1.3 | 1.2 | + | A |
| Oncostatin M (Osm)b | D31942 | 8.1 | 2.5 | 0.9 | 1.2 | + | A |
| Helix-loop-helix DNA binding protein (Id) | M31885 | 5 | 9.3 | 9.2 | 4.9 | + | A |
| p21 (WAF1/CIP1)b | NM_007669 | 4.1 | 1.1 | 2.3 | 1.8 | + | A |
| Fos-like antigen 2 (FosL2/Fra-2) | NM_008037 | 3.8 | 1.2 | 1.1 | 0.7 | + | A |
| Serine protease inhibitor 2-1 (Spi-2.1)b | M64085 | 3 | 0.8 | 1.4 | 1.9 | + | A |
| Fatty acid synthase | AF127033 | 1.7 | 4.9 | 2.6 | 3.8 | + | A |
| IL-2 receptor alphab | NM_008367 | 1.1 | 4 | 0.9 | 1.5 | + | A |
| NIFK (nucleolar RNA binding protein) | AB056870 | 1.5 | 3.5 | 2.1 | 2 | + | A |
| T-cell receptor gamma variable 4 (TCRγ-V4)b | NM_011558 | 1.3 | 3.4 | 0.5 | 0.3 | + | A |
| Thrombin receptor (Cf2r) | U36757 | 2.1 | 3.4 | 1.2 | 1.9 | + | A |
| Downstream of tyrosine kinase 2 (dok2) | NM_010071 | 1.3 | 3 | 1 | 0.5 | + | A |
| TDAG51/PQ | NM_009344 | 5.2 | 2.8 | 2.6 | 2.3 | − | C |
| Mitogen-activated protein kinase phosphatase 1 (MKP-1) | X61940 | 4 | 1 | 0.9 | 1 | − | C |
| IL-4 receptor alpha | M29854 | 3.4 | 6.5 | 2.6 | 3.6 | − | C |
| Stimulated by retinoic acid 13 (stra13/clast5) | AF010305 | 3.2 | 3.7 | 0.9 | 0.7 | − | C |
| Proprotein convertase subtilisin/kexin, type 3 (Furin) | NM_011046 | 2.2 | 4 | 1.5 | 1.4 | − | C |
| Receptor-like tyrosine kinase (ryk)e | M98547 | 1.7 | 6.2 | 1.2 | 1 | ? | D |
| Similar to phosphatidylinositol binding clathrin assembly proteine | BC021491 | 1.3 | 4.1 | 1.7 | 1.6 | ? | D |
| Unaffected by TSA | |||||||
| Immediate-early response 2 (ier2) | NM_010499 | 7.4 | 2.4 | 10.4 | 5.3 | − | B |
| B-cell translocation gene 2 (btg2/tis21) | NM_007570 | 5.2 | 0.6 | 5.7 | 2 | − | B |
| Phosphatidylinositol transfer protein beta | U46934 | 4.6 | 4 | 6.9 | 6 | − | B |
| JunB | XM_125115 | 4.4 | 2.2 | 6.2 | 2 | − | B |
| Cytoskeletal gamma-actin | M21495 | 4.1 | 3.7 | 3.1 | 3.1 | − | B |
| Cytoskeletal beta-actin | X03672 | 3.2 | 3.4 | 2.1 | 2.4 | − | B |
| DEAD/H box polypeptide 21 (RNA helicase II/Gu) | NM_019553 | 1.2 | 4.1 | 1.9 | 4.5 | − | B |
| Secreted phosphoprotein 1 (Spp1/Osteopontin) | NM_009263 | 1.3 | 3.3 | 1.4 | 2.8 | − | B |
Genes induced 3- to 10-fold by IL-3 at 30 min and/or 2 h (n = 28). Twenty genes were downregulated 1.8- to 10.1-fold, by TSA, and eight genes were unaffected by TSA.
Known STAT5 target genes from the literature.
Induction relative to that of untreated unstimulated cells. No significant differences were found between the untreated and the TSA-treated unstimulated cells.
STAT5 targets were identified by real-time PCR from unstimulated and IL-3-stimulated Ba/F3-1*6 cells that express a constitutively active form of STAT5A (see text).
Genes that lost IL-3 inducibility in the Ba/F3-1*6 cell line and thus could not be identified as STAT5 or non-STAT5 targets.