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. 2003 Jun;23(12):4344–4355. doi: 10.1128/MCB.23.12.4344-4355.2003

FIG. 4.

FIG. 4.

Polysome analysis. Extracts were prepared from mid-log-phase cultures and sedimented through 7 to 47% sucrose gradients. Gradients were analyzed by monitoring the absorbance at 254 nm. Arrows indicate reduced free 60S peaks. (A) Wild-type (CH1305, WT) and the glucose-repressible (AJY1118, GAL::NOG1) strains were cultured in galactose-containing medium. Glucose was added to 2%, and the cells were harvested after an additional 8 h. (B) AJY1124 (GAL1::NOG1) containing pAJ633 (nog1-1) or pAJ637 (nog1-3) were grown to log phase at 30°C, followed by incubation at a restrictive temperature (37°C) for 4 h. (C) AJY1167 (LSG1::KanMX) containing pAJ740 (lsg1-1) or pAJ741 (lsg1-2) were cultured as described for the nog1 mutants in panel B.