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. 2003 Jun;23(12):4344–4355. doi: 10.1128/MCB.23.12.4344-4355.2003

FIG. 9.

FIG. 9.

Rpl25-eGFP localization. (A) Rpl25-eGFP was expressed in strain CH1305 (wild-type), strain AJY1124 (GAL1::NOG1) carrying pAJ637 (nog1-3), and strain AJY1167 (LSG1::KanMX) carrying pAJ740 (lsg1-1). Fresh overnight cultures were diluted twofold into fresh medium. After 30 min at 30°C, each culture was divided, and one half of each shifted to 37°C for 3 h. Enlarged examples compare the localization of the Rpl25-eGFP with that of DNA stained with Hoechst 34442. For the colocalization experiments, the strains were AJY1124 (GAL1::NOG1) carrying pAJ633 (nog1-1) and AJY1167 (LSG1::KanMX) carrying pAJ740 (lsg1-1). GFP fluorescence (green) and Hoechst fluorescence (red) were visualized in separate channels, artificially colored, and merged. (B) Rpl25-eGFP was expressed in AJY734 (nmd3-4) at a permissive (30°C) or a nonpermissive (37°C) temperature for 3 h or coexpressed in CH1305 containing pAJ368 (GAL1::NMD3Δ100) (20) in the presence of the inducer galactose (gal) or in the presence of the noninducing sugar raffinose (raf). Enlarged examples of cells are shown.