FIG. 2.
Construction and transcription of an H4 minigene. (A) Diagram of H4 gene and derived minigene template. The minigene template generated by PCR contained a biotin molecule on the upstream end and was missing 328 nt from the coding region of the H4 gene. (B) Pulse-chase assay with H3 minigene template and Kc cell nuclear extract. RNA was isolated at the indicated times and analyzed on a 6.7% denaturing gel, followed by autoradiography. M, DNA markers of indicated sizes. Runoff (RO), end-labeled tRNAs, and processed transcript (arrow) are indicated.