FIG. 3.
Kinetic analysis of transcription and processing from the H4 minigene template. (A) Transcription from isolated early elongation complexes. Early elongation complexes were generated and isolated as described in Materials and Methods and then allowed to elongate with or without Kc cell nuclear extract as indicated. RNA was isolated at the indicated times and analyzed on a 6.7% denaturing gel, followed by autoradiography. A, arrested transcript; arrow, processed H4 minigene RNA. (B) Quantitation of processed transcript. The processed transcript was quantified by using a Packard InstantImager, and the relative counts were plotted.