FIG. 6.

Dependence of processing on extract concentration. Free RNA (A) or isolated elongation complexes (B) were processed by using nuclear extract for the indicated times. A constant amount of extract was used for each reaction, but the concentration was changed by dilution as indicated. RNA was isolated at the indicated times and analyzed on a 6.7% denaturing gel, followed by autoradiography. A, arrested transcript; arrow, processed transcript. The percentage of total RNA processed is given under each lane.