FIG. 4.

Effect of site-directed mutagenesis on the ORF34 promoter in response to hypoxia. (A) Sequences of the wild-type (WT) and HRE-2 mutant ORF34D1 reporters. The mutant reporter has a 3-bp substitution within the HRE-2 sequence, which is shown in bold. (B) Cells were cotransfected with a wild-type or mutant reporter and normalized as described in the legend to Fig. 1. All values represent means of triplicate determinations in one representative experiment out of three, with error bars denoting the standard deviations. ***, P < 0.0001 compared with normoxia.