FIG. 1.

SV5 is a poor inducer of IL-8 and MCP-1 secretion compared to other closely related rubulaviruses. (A) Monolayers of A549 cells were mock infected or were infected with WT rSV5, HPIV-2, WF-PIV, SV41, or HPIV-3. At 22 hpi media were harvested and analyzed by ELISA for IL-8. Values are the mean concentration of triplicate samples of IL-8 in picograms per milliliter, with standard deviations shown by bars, and are normalized to 10⁶ cells. (B and C) Time course of chemokine secretion. A549 cells were mock infected or were infected with rSV5 and HPIV-2 at a high MOI. At the indicated times postinfection media were harvested and assayed by ELISA for IL-8 (B) or MCP-1 (C). Results are representative of three experiments.