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Stability of WT and R101A and R105K mutated M1 proteins. At 18 h posttransfection, 293T cells were pulse labeled for 2 h (lanes P) and then chased with excess Met and Cys for 3 h (lanes C). Cells were then lysed in RIPA buffer, immunoprecipitated with anti-M1 antibody, and analyzed by SDS-PAGE.
