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. 2003 Jun;77(12):6785–6798. doi: 10.1128/JVI.77.12.6785-6798.2003

FIG. 4.

FIG. 4.

Rep-210-mediated resistance to TYLCV-[PT] and TYLCSV involves two different mechanisms.(A) Southern blot of TNA extracts from wild-type (wt) or transgenic Rep-210 expressing (line 102.22) N. benthamiana protoplasts transfected with infectious clones of TYLCSV or TYLCV-[PT], as indicated. The values 1/20 and 1/100 represent the dilution factors of loaded samples. The blot was probed with a digoxigenin-labeled TYLCSV Rep-210 sense RNA probe (S) and reprobed with a digoxigenin-labeled TYLCV-[PT] V1 antisense RNA probe (PT). (B) GUS activity in transgenic Rep-210 expressing N. benthamiana protoplasts (line 102.22 [solid bars]) transfected with the leftmost indicated plasmids or wild-type N. benthamiana protoplasts (gray bars) cotransfected with the leftmost indicated plasmids together with the plasmids indicated on the left. For each GUS-fused plasmid, the GUS activity in wild-type protoplasts was taken as 100%. Each value is the average of three transfections of three independent experiments. Error bars indicate the standard error of the mean.