TABLE 1.
Constructs used in this study
| Name | Viral sequencesa | Viral DNA coordinatesb |
|---|---|---|
| TYLCSV expression cassettes | ||
| pJTR359 (previously pTOM120)c | 42-bp UTR + C1 ORF | 1538-2656 (1) |
| pJTR210a (previously pTOM100)c | 42-bp UTR + truncated C1 ORF (630 nt) containing C4 coding region | 1985-2656 (1) |
| pJTR210 (previously pTOM100C4-)c | 42-bp UTR + truncated C1 ORF (630 nt) | 1985-2656 (1) |
| pJTR181 | 42-bp UTR + truncated C1 ORF (543 nt) | 2072-2556 (1) |
| pJTR156 | 42-bp UTR + truncated C1 ORF (468 nt) | 2147-2656 (1) |
| pJTR130 | 42-bp UTR + truncated C1 ORF (390 nt) | 2225-2656 (1) |
| pJTR120 | 42-bp UTR + truncated C1 ORF (360 nt) | 2255-2656 (1) |
| pJTR80 | 42-bp UTR + truncated C1 ORF (240 nt) | 2375-2656 (1) |
| pJTR54 | 42-bp UTR + truncated C1 ORF (162 nt) | 2453-2656 (1) |
| pTOM130 | KpnI-BglII cassette of pJTR130 in the binary vector pBIN19 | 2225-2656 (1) |
| Yeast two-hybrid plasmids | ||
| pASPTRep-359 | GAL4 BD fused to TYLCV-[PT] Rep | 1551-2630 (2) |
| pASRep-359 | GAL4 BD fused to TYLCSV Rep | 1538-2614 (1) |
| pASRep-210 | GAL4 BD fused to TYLCSV Rep210 | 1985-2614 (1) |
| pASRep-181 | GAL4 BD fused to TYLCSV Rep181 | 2072-2614 (1) |
| pASRep-156 | GAL4 BD fused to TYLCSV Rep156 | 2147-2614 (1) |
| pASRep-130 | GAL4 BD fused to TYLCSV Rep130 | 2225-2614 (1) |
| pASRep-120 | GAL4 BD fused to TYLCSV Rep120 | 2255-2614 (1) |
| pASRep-80 | GAL4 BD fused to TYLCSV Rep80 | 2375-2614 (1) |
| pASSNF1 | GAL4 BD fused to SNF1 | |
| pACTPTRep-359 | GAL4 AD fused to TYLCV-[PT] Rep | 1551-2630 (2) |
| pACTRep-359 | GAL4 AD fused to TYLCSV Rep | 1538-2614 (1) |
| pACTRep-210 | GAL4 AD fused to TYLCSV Rep210 | 1985-2614 (1) |
| pACTRep-181 | GAL4 AD fused to TYLCSV Rep180 | 2072-2614 (1) |
| pACTRep-156 | GAL4 AD fused to TYLCSV Rep156 | 2147-2614 (1) |
| pACTRep-130 | GAL4 AD fused to TYLCSV Rep130 | 2225-2614 (1) |
| pACTRep-120 | GAL4 AD fused to TYLCSV Rep120 | 2255-2614 (1) |
| pACTRep-80 | GAL4 AD fused to TYLCSV Rep80 | 2375-2614 (1) |
| pACTSNF4 | GAL4 AD fused to SNF4 | |
| S. pombe plasmids | ||
| p42Rep-359 | HIS tag fused to TYLCSV Rep | 1538-2614 (1) |
| p42Rep-210 | HIS tag fused to TYLCSV Rep210 | 1985-2614 (1) |
| p42Rep-181 | HIS tag fused to TYLCSV Rep181 | 2072-2614 (1) |
| p42Rep-156 | HIS tag fused to TYLCSV Rep156 | 2147-2614 (1) |
| p42Rep-130 | HIS tag fused to TYLCSV Rep130 | 2225-2614 (1) |
| p41Rep-359 | HA tag fused to TYLCSV Rep | 1538-2614 (1) |
| p41Rep-210 | HA tag fused to TYLCSV Rep210 | 1985-2614 (1) |
| p41Rep-181 | HA tag fused to TYLCSV Rep181 | 2072-2614 (1) |
| p41Rep-156 | HA tag fused to TYLCSV Rep156 | 2147-2614 (1) |
| p41Rep-130 | HA tag fused to TYLCSV Rep130 | 2225-2614 (1) |
| GUS reporter plasmids | ||
| pTOM202c | IR of TYLCSV; translational fusion of C1 promoter to GUS gene | 2600-152 (1) |
| pIntS/GUSc | IR of TYLCSV; transcriptional fusion of C1 promoter to GUS gene | 2615-152 (1) |
| pIntS-ES[1]/GUSc | IR of TYLCSV-ES[1]; transcriptional fusion of C1 promoter to GUS gene | 2620-154 (3) |
| pIntPT/GUS | IR of TYLCV-[PT]; transcriptional fusion of C1 promoter to GUS gene | 2629-163 (2) |
| Infectious TYLCSV DNA clones | ||
| pTOM6 | SacI tandem dimer of TYLCSV menome | |
| pSP97 | SpeI-BamHI 1.8-mer of TYLCSV-ES[1] genome | |
| pPORT | SphI-BamHI 1.7-mer of TYLCV-[PT] genome |