TYLCSV expression cassettes |
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pJTR359 (previously pTOM120)c
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42-bp UTR + C1 ORF |
1538-2656 (1) |
pJTR210a (previously pTOM100)c
|
42-bp UTR + truncated C1 ORF (630 nt) containing C4 coding region |
1985-2656 (1) |
pJTR210 (previously pTOM100C4-)c
|
42-bp UTR + truncated C1 ORF (630 nt) |
1985-2656 (1) |
pJTR181 |
42-bp UTR + truncated C1 ORF (543 nt) |
2072-2556 (1) |
pJTR156 |
42-bp UTR + truncated C1 ORF (468 nt) |
2147-2656 (1) |
pJTR130 |
42-bp UTR + truncated C1 ORF (390 nt) |
2225-2656 (1) |
pJTR120 |
42-bp UTR + truncated C1 ORF (360 nt) |
2255-2656 (1) |
pJTR80 |
42-bp UTR + truncated C1 ORF (240 nt) |
2375-2656 (1) |
pJTR54 |
42-bp UTR + truncated C1 ORF (162 nt) |
2453-2656 (1) |
pTOM130 |
KpnI-BglII cassette of pJTR130 in the binary vector pBIN19 |
2225-2656 (1) |
Yeast two-hybrid plasmids |
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pASPTRep-359 |
GAL4 BD fused to TYLCV-[PT] Rep |
1551-2630 (2) |
pASRep-359 |
GAL4 BD fused to TYLCSV Rep |
1538-2614 (1) |
pASRep-210 |
GAL4 BD fused to TYLCSV Rep210 |
1985-2614 (1) |
pASRep-181 |
GAL4 BD fused to TYLCSV Rep181 |
2072-2614 (1) |
pASRep-156 |
GAL4 BD fused to TYLCSV Rep156 |
2147-2614 (1) |
pASRep-130 |
GAL4 BD fused to TYLCSV Rep130 |
2225-2614 (1) |
pASRep-120 |
GAL4 BD fused to TYLCSV Rep120 |
2255-2614 (1) |
pASRep-80 |
GAL4 BD fused to TYLCSV Rep80 |
2375-2614 (1) |
pASSNF1 |
GAL4 BD fused to SNF1 |
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pACTPTRep-359 |
GAL4 AD fused to TYLCV-[PT] Rep |
1551-2630 (2) |
pACTRep-359 |
GAL4 AD fused to TYLCSV Rep |
1538-2614 (1) |
pACTRep-210 |
GAL4 AD fused to TYLCSV Rep210 |
1985-2614 (1) |
pACTRep-181 |
GAL4 AD fused to TYLCSV Rep180 |
2072-2614 (1) |
pACTRep-156 |
GAL4 AD fused to TYLCSV Rep156 |
2147-2614 (1) |
pACTRep-130 |
GAL4 AD fused to TYLCSV Rep130 |
2225-2614 (1) |
pACTRep-120 |
GAL4 AD fused to TYLCSV Rep120 |
2255-2614 (1) |
pACTRep-80 |
GAL4 AD fused to TYLCSV Rep80 |
2375-2614 (1) |
pACTSNF4 |
GAL4 AD fused to SNF4 |
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S. pombe plasmids |
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p42Rep-359 |
HIS tag fused to TYLCSV Rep |
1538-2614 (1) |
p42Rep-210 |
HIS tag fused to TYLCSV Rep210 |
1985-2614 (1) |
p42Rep-181 |
HIS tag fused to TYLCSV Rep181 |
2072-2614 (1) |
p42Rep-156 |
HIS tag fused to TYLCSV Rep156 |
2147-2614 (1) |
p42Rep-130 |
HIS tag fused to TYLCSV Rep130 |
2225-2614 (1) |
p41Rep-359 |
HA tag fused to TYLCSV Rep |
1538-2614 (1) |
p41Rep-210 |
HA tag fused to TYLCSV Rep210 |
1985-2614 (1) |
p41Rep-181 |
HA tag fused to TYLCSV Rep181 |
2072-2614 (1) |
p41Rep-156 |
HA tag fused to TYLCSV Rep156 |
2147-2614 (1) |
p41Rep-130 |
HA tag fused to TYLCSV Rep130 |
2225-2614 (1) |
GUS reporter plasmids |
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pTOM202c
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IR of TYLCSV; translational fusion of C1 promoter to GUS gene |
2600-152 (1) |
pIntS/GUSc
|
IR of TYLCSV; transcriptional fusion of C1 promoter to GUS gene |
2615-152 (1) |
pIntS-ES[1]/GUSc
|
IR of TYLCSV-ES[1]; transcriptional fusion of C1 promoter to GUS gene |
2620-154 (3) |
pIntPT/GUS |
IR of TYLCV-[PT]; transcriptional fusion of C1 promoter to GUS gene |
2629-163 (2) |
Infectious TYLCSV DNA clones |
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pTOM6 |
SacI tandem dimer of TYLCSV menome |
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pSP97 |
SpeI-BamHI 1.8-mer of TYLCSV-ES[1] genome |
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pPORT |
SphI-BamHI 1.7-mer of TYLCV-[PT] genome |
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