Table 1.
Axon outgrowth and guidance defects in unc-69 mutants
| ALM defect (%) | ||||||
| Genotype | B | NR | E | FL | n | |
| Wild type | 0 | 0 | 0 | 12 | 113 | |
| unc-69(e602) | 12 | 36 | 77 | 84 | 77 | |
| unc-69(e587) | 15 | 45 | 85 | 89 | 80 | |
| unc-69(e602) (m+z-) | 0 | 4.3 | 39 | 91 | 70 | |
| unc-69(e602)/nDf40 (m+z-) | 0 | 7.2 | 20 | 72 | 69 | |
| unc-69(e587) (m+z-) | 1.4 | 7.2 | 43 | 87 | 69 | |
| unc-69(e587)/nDf40 (m+z-) | 0 | 8.3 | 20 | 82 | 60 | |
| unc-69(e602)* | 19 | 48 | 62 | 95 | 113 | |
| unc-69(e602); opEx [P mec -7 ::unc-69]* | 1 | 12 | 2 | 5 | 81 | |
| unc-69(e602); opEx [P mec -7 ::unc-69]* | 5 | 9 | 6 | 10 | 79 | |
| unc-69(e602); opEx [P mec -7 ::unc-69]* | 8 | 29 | 0 | 10 | 85 | |
| AVM defect (%) | ||||||
| Genotype | B | NR | E | FL | V | n |
| Wild type | 0 | 0 | 1 | 8 | 0 | 106 |
| unc-69(e602) | 32 | 27 | 72 | 73 | 2.7 | 77 |
| unc-69(e587) | 64 | 70 | 86 | 87 | 0 | 80 |
| unc-69(e602) (m+z-) | 1.4 | 4.3 | 57 | 86 | 0 | 70 |
| unc-69(e602)/nDf40 (m+z-) | 0 | 0 | 56 | 80 | 0 | 69 |
| unc-69(e587) (m+z-) | 2.9 | 8.8 | 56 | 85 | 0 | 69 |
| unc-69(e587)/nDf40 (m+z-) | 0 | 3.6 | 76 | 95 | 0 | 60 |
| unc-69(e602)* | 46 | 67 | 93 | 100 | ND | 113 |
| unc-69(e602); opEx [P mec -7 ::unc-69]* | 0 | 4 | 4 | 4 | ND | 81 |
| unc-69(e602); opEx [P mec -7 ::unc-69]* | 8 | 12 | 12 | 23 | ND | 79 |
| unc-69(e602); opEx [P mec -7 ::unc-69]* | 11 | 21 | 12 | 13 | ND | 85 |
Neurite outgrowth and guidance defects of mechanosensory touch neurons in unc-69 mutants. The morphology of neurites of ALM (top) and AVM (bottom) neurons (as in the schematic in Figure 2) was scored in different unc-69 mutants, in unc-69/nDf40 heterozygotes, and in mosaic animals carrying a functional unc-69 transgene under the control of the mec-7 promoter, which directs expression in the six touch neurons. All worms scored had a Pmec-4::gfp transgene zdIs5 in the background to allow visualization of the neurite morphology. One ALM neurite was scored per animal. B, failure to form proper branch at the nerve ring; NR, failure of nerve ring branch to fully extend; E: failure to elongate past the branch point; FL, failure to extend fully; V, ventral guidance defect. (m+z-): homozygous mutant animals derived from heterozygous mothers. *These strains also carry a lin-15(n765) mutation in the background. All opEx transgenes also carry a wild-type copy of lin-15(+) as a coinjection marker. ND, not done. n, number of worms scored.