FIG. 2.

Human EBP2 causes EBNA1 to attach to yeast chromosomes. Chromatin from G₁ or mitotic yeast cells expressing human EBP2 (hEBP2), EBNA1, or human EBP2 and EBNA1 were spread on slides, washed to remove proteins not bound to DNA, and stained with antibodies against human EBP2 and either EBNA1 or NOP1. DNA was visualized by DAPI staining. Each image contains chromatin from two yeast cells. Immunofluorescence microscopy was performed under identical conditions.