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Ability of human EBP2 domains fused to 21K to mediate plasmid partitioning. Plasmid loss assays were used to determine the stability of YRp7FR in the presence of a plasmid expressing human EBP2 (hEBP2), human EBP2 fused to 21K, or the indicated human EBP2 domains fused to 21K. After 11 generations in the absence of selection for YRp7FR, diluted cultures were plated on selective and nonselective plates with respect to YRp7FR. Schematic diagrams of the proteins are shown on the left.
