FIG. 6.

Middle coiled-coil domain of human EBP2 binds human mitotic chromosomes. GFP, GFP fused to human EBP2 (GFP/hEBP2), and GFP fused to human EBP2 fragments 1 to 100 (GFP/N), 95 to 220 (GFP/M), 220 to 306 (GFP/C), 1 to 220 (GFP/NM), and 95 to 306 (GFP/MC) were tested for their ability to bind mitotic chromosomes in HeLa cells. The mitotic chromosomes were visualized with DAPI, and the localization of GFP-containing proteins was determined by fluorescence microscopy. Images were captured with similar exposure times.