FIG. 8.

Nucleolar localization of yeast EBP2 and yeast EBP2-based fusion proteins. Log-phase yeast cells (left panel) and spreads of yeast mitotic chromosomes (right panel) are shown for S. cerevisiae expressing endogenous yeast EBP2 (yEBP2), overexpressed yeast EBP2 (yEBP2 O/E), or a yeast EBP2-based fusion protein (F1 to F5). Cells and chromosome spreads were stained for the EBP2 proteins and for Nop1 (as a nucleolar marker) and counterstained with DAPI. Images were captured by immunofluorescence microscopy under identical conditions.