TABLE 2.
Proteaseb | Mean Cleavage efficiency (%) ± SD
|
|
---|---|---|
HIV RT/IN peptide (RKIL/FLDG) | Phage library peptide (SGIM/FESN) | |
Wild-type FN | 16 ± 2 | 8 ± 1 |
Mutants | ||
37/55/56/59 | 76 ± 9 | 33 ± 4 |
55/56/59/99 | 28 ± 5 | 30 ± 6 |
37/55/56/59/99 | 60 ± 8 | 51 ± 7 |
37/55/56/59/98/99 | 82 ± 8 | 42 ± 5 |
F9s | 72 ± 11 | 36 ± 6 |
Wild-type HIV-1 | 81 ± 10 | 93 ± 7 |
The substrate specificity was analyzed by assaying the cleavage efficiency of mutants on two peptides. These two peptides were very efficiently cleaved by HIV-1 PR but not by FIV PR. The assay was done with 100 nM PR and 10 min of incubation for the HIV RT/IN junction peptide and 75 nM PR and 5 min of incubation for the phage library peptide. The data are the means ± standard deviation of three independent experiments.
See Table 1, footnote a.