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. 2003 Jun;77(12):6589–6600. doi: 10.1128/JVI.77.12.6589-6600.2003

TABLE 2.

Altered substrate specificity of chimeric FIV PRs on peptidesa

Proteaseb Mean Cleavage efficiency (%) ± SD
HIV RT/IN peptide (RKIL/FLDG) Phage library peptide (SGIM/FESN)
Wild-type FN 16 ± 2 8 ± 1
Mutants
    37/55/56/59 76 ± 9 33 ± 4
    55/56/59/99 28 ± 5 30 ± 6
    37/55/56/59/99 60 ± 8 51 ± 7
    37/55/56/59/98/99 82 ± 8 42 ± 5
    F9s 72 ± 11 36 ± 6
Wild-type HIV-1 81 ± 10 93 ± 7
a

The substrate specificity was analyzed by assaying the cleavage efficiency of mutants on two peptides. These two peptides were very efficiently cleaved by HIV-1 PR but not by FIV PR. The assay was done with 100 nM PR and 10 min of incubation for the HIV RT/IN junction peptide and 75 nM PR and 5 min of incubation for the phage library peptide. The data are the means ± standard deviation of three independent experiments.

b

See Table 1, footnote a.