FIG. 2.

The epitope PR82V_76-84 is restricted by HLA-A2. The HLA-A2 restriction of PR82V_76-84 was confirmed in a killing assay using HLA-A2-matched and -mismatched BCL presenting PR82V_76-84. An antigen-specific CTL line had been established from patient 3156 by peptide stimulation of fresh PBMC for 14 days. The class I HLA haplotypes of the matched BCL were BCL-1 (HLA-A2/30 [19]), B7/65 (Cw7/8 [15]), and BCL-2 (HLA-A2/11, B35/53, Cw4/6), and for the mismatched BCL-3 they were HLA-A1, B7, Cw7, and BCL-4 A30 (19)/74 (19), Cw2/7. The effector-to-target ratio was 30:1. The background killing ranged from 0 to 5% (median 0%) as determined by lysis of unpulsed BCL.