FIG. 3.
Expression of GFP-tagged C2 protein and PVX coat protein in N. benthamiana plants. Proteins were extracted from leaves of mock-inoculated plants (lane 1) and plants infected with PVX/GFP (lane 2), PVX/C2-GFP (lane 3), PVX/C21-31-GFP (lane 4), PVX/C217-31-GFP (lane 5), PVX/C2-K17D-GFP (lane 6), PVX/C2-HR21DV-GFP (lane 7), PVX/C2-KK25DI-GFP (lane 8), and PVX/C2-RRRR31DVGG-GFP (lane 9) and harvested 13 days p.i. Proteins were detected by Western blot analysis with antibodies specific to (A) PVX coat protein (A) and GFP (B). The positions and sizes of protein markers, the PVX coat protein, GFP, and GFP-tagged C2 protein are indicated.