TABLE 2.
Construction of C2 expression cassettes
| Constructa | Primer pair (5′ region/3′ region) | Cloning sites | Mutation |
|---|---|---|---|
| PVX/C21-31-GFP | PP47-PP115 | ClaI-EagI | C21-31 |
| PVX/C217-31-GFP | PP125-PP126 | ClaI-EagI | C217-31 |
| PVX/C2-K17D-GFP | PP47-PP116/PP117-PP49 | ClaI-AatII + AatII-EagI | Lys17 → Asp |
| PVX/C2-HR21DV-GFP | PP47-PP118/PP119-PP49 | ClaI-AatII + AatII-EagI | His20 → Asp, Arg21 → Ala |
| PVX/C2-KK25DI-GFP | PP47-PP120/PP121-PP49 | ClaI-AatII + AatII-EagI | Lys24 → Asp, Lys25 → Ileu |
| PVX/C2-RRRR31DVGG-GFP | PP47-PP122/PP123-PP49 | ClaI-AatII + AatII-EagI | Arg28 → Asp, Arg29 → Val, Arg30 → Gly, Arg31 → Gly |
a
The PVX/GFP vector was used for all constructs.