FIG. 3.

Localization of CD8-HIV chimeric molecules by confocal microscopy. HeLa cells were cotransfected with pCD8-HIV with either pSRα-STVSVG (a to c) or pSFFI-gE (d to f). The transfected cells were fixed and processed for immunofluorescence with the CD8-Leu2A MAb (a and d) and antibodies against ST (b) or VZV gE (e). Before fixation, all the cells were treated with 50 μg of cycloheximide/ml for 3 h. Colocalization was examined by confocal microscopy as described in the legend to Fig. 1. Scale bar, 20 μm.