FIG. 8.

Expression of Env mutated in Y₈₀₂W₈₀₃ at the cell surface. (A) Identical quantities of lysates from transfected cells were analyzed by Western blotting with anti-Env MAb. NT, untransfected control cells. (B) 293T cells (2 × 10⁶) were cotransfected by the calcium phosphate method with 8 μg of wt (thick line) or Y₈₀₂W₈₀₃-SL (thin line) Env expression vector and 0.5 μg of pRSV-GFP vector. The amount of Env at the surface of GFP⁺ cells was analyzed by flow cytometry 48 h later. Dotted line, cells transfected with the pRSV-GFP vector alone, as the negative control (ctrl). Data are representative of three independent experiments.