FIG. 9.

Replication in Jurkat T cells of wt and Y₈₀₂W₈₀₃-SL mutant HIV-1 virus. (A) Expression of Gag (p160Gag-Pol, p41, p55Gag, or CAp24) proteins of wt and Y₈₀₂W₈₀₃-SL mutant HXB2 HIV-1 provirus in transfected 293T cells was verified by Western blot analysis with anti-p24 antibodies. NT, untransfected cells. (B) Replication kinetics. The Jurkat T-cell line was infected in parallel with a low inoculum of wt and Y₈₀₂W₈₀₃-SL mutant HXB2 HIV-1 (50 ng of p24/10⁶ cells). Cells were split every 2 or 3 days; culture supernatant samples were collected at each point for p24 antigen quantification. Values are the averages of 3 independent experiments ± standard deviations. CTRL, noninfected cells. (C) Sequence comparison for residues 797 to 812 of the Env cytosolic domain of the different HIV-1 subgroups. The amino acid sequences of representative isolates of different clades (21) (A, U455; B, HXB2R; C, ETH2220; D, ELI; AE, 92TH011A; F1, 93BR020.1; GH, AG-VI525A2; H, 90CR056; O, ANT70) of the HIV-1 groups M, N, and O were aligned. YW is shown in boldface.