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. 2003 Jun;77(12):6979–6987. doi: 10.1128/JVI.77.12.6979-6987.2003

TABLE 1.

VZV PCR of separated neurons and satellite cells

Specimena Age (yr)/sexb Cause of death Time from death to autopsy (h)g Results for:
Neurons
Satellite cells
No. of tubes testedd No. of cells tested No. positive Latent % of fractionh No. of tubes tested Total no. of clumps Total no. of cellse No. positive
HG-70 71/M CHFc 6 2 26 1 3.8 13 46 2,484 0
HG-76 90/F Pneumonia 3 34 340 4 1.2 18 38 2,052 0
HG-79 49/F Carcinoma 14 22 220 6 2.7 18 90 4,860 0
HG-82 85/M Lymphoma 16 22 220 2 0.9 6 30 1,620 0
HG-83 64/M Alzheimer's Unknown 12 120 1 0.8 NDf
HG-88 66/M Chronic leukemia 15 18 180 3 1.7 18 90 4,860 0
HG-91 72/M Peritonitis 21 22 220 3 1.4 18 90 4,860 0
HG-93 28/M Lymphoma 18 18 180 4 2.2 ND
HG-95 66/F CHF 6 18 180 2 1.1 ND
HG-97 44/M Cystic fibrosis 22 18 180 3 1.7 ND
HG-100 59/M CHF/diabetes 16 18 180 3 1.7 ND
HG-101 61/M Chronic leukemia 20 18 180 2 1.1 ND
Total 222 2,226 34 91 384 20,736 0
a

A fragment of each ganglion was demonstrated by PCR to contain VZV DNA prior to cell separation. Serum from each autopsy contained VZV-specific antibody.

b

Numbers indicate age at death (average, 62.9 years). M, male; F, female.

c

CHF, congestive heart failure.

d

Contained 10 neurons per tube except for HG-70 (10 and 16 cells).

e

Each clump of satellite cells contained a mean of 54 cells (21).

f

ND, not done.

g

Average, 14.3 h.

h

Average ± standard deviation, 1.7% ± 0.86%.