FIG. 2.

Characterization of recombinant vaccinia viruses by Southern blotting. Genomic DNA of the different viruses was digested with the respective restriction endonuclease, transferred to a filter, and hybridized to the indicated radioactively labeled probes. (A) Viral DNAs hybridized to the gag-pol probe showed the expected MunI fragments of 6.9 and 4.5 kb in the gag-pol, packaging, and triple viruses (lanes 2 to 5). (B) Hybridization with the VSV-G probe revealed the characteristic 5.5-kb Asp718 fragments in the packaging and triple viruses (lanes 3 to 5). (C) The 7.2-kb XhoI fragment hybridizing with the EGFP probe was found only in the triple viruses (lanes 4 and 5). The wild-type virus (lanes 1) did not show these signals. The numbers at the left show the marker bands; those at the right show the sizes of the fragments (in kilobase pairs).