TABLE 2.
PERT assays for detection of replication-competent retrovirusa
| Group | Sample | Reverse transcriptase (nU/ml) | Titer (CFU/ml) |
|---|---|---|---|
| Positive and negative controls | Lys CV-1 mock | <2 | 0 |
| Sup CV-1 mock | <2 | 0 | |
| Sup CV-1/wt virus | <2 | 0 | |
| Sup CV-1/triple virus#9 | 211,046 | 104 | |
| Lys PT67/mock | 4,405 | 0 | |
| Sup PT67/mock | 86,234 | 0 | |
| Sup PT67/wt virus | 338,517 | 0 | |
| Sup PT67/triple virus | 6,584 | 103 | |
| Transduced 3T3 supernatants | Sup 3T3 (CV-1/triple virus 9) | <2 | ND |
| Sup 3T3 (CV-1/wt virus) | <2 | ND | |
| Lys 3T3 mock | 13 | 0 | |
| Sup 3T3 mock | <2 | 0 |
a
wt, wild type; lys, cell lysate; sup, supernatant; ND, not determined.