TABLE 1.
Titers for rAAAV, rAAV2, rAAV4, and rAAV5 expressing LacZ in avian and mammalian cell lines and primary cells
| Cell type | Transducing units per 106 genomesa
|
|||
|---|---|---|---|---|
| rAAAV | rAAV2 | rAAV4 | rAAV5 | |
| CEF | 7,140 ± 380 | 25 ± 3.5 | 84 ± 6.3 | 58 ± 5.7 |
| DF-1 | 530 ± 35 | 8 ± 0.9 | 45 ± 4.7 | 60 ± 6.1 |
| LMH | 2,380 ± 145 | 230 ± 25 | 34 ± 5.6 | 40 ± 4.9 |
| DT-90 | ND | ND | ND | ND |
| QNR | 1,260 ± 90 | 176 ± 18 | 42 ± 5.2 | 185 ± 26 |
| QT6 | 930 ± 62 | 112 ± 21 | 23 ± 3.8 | 33 ± 5 |
| Chicken primary embryonic kidney cells | 8,080 ± 560 | 422 ± 46 | 350 ± 40 | 235 ± 38 |
| Chicken primary pituitary cells | 4,640 ± 375 | 144 ± 17 | 70 ± 12 | 91 ± 8.4 |
| 293T | ND | 4,500 ± 355 | 3,130 ± 270 | 684 ± 57 |
| COS | 5 ± 0.7 | 6,920 ± 420 | 3,550 ± 165 | 592 ± 53 |
| A549 | ND | 2,190 ± 315 | 1,360 ± 140 | 26 ± 4.3 |
| Human primary fibroblasts | ND | 1,990 ± 170 | 1,130 ± 145 | 292 ± 31 |
a
Transductions were performed as described in Materials and Methods, and efficiency is expressed as transducing units per 106 recombinant particles. Numbers represent mean ± standard deviation from four independent assays. ND, none detected.