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Effects of α-defensin-1 pretreatment of cells on HIV-1 infection. HeLa-CD4 cells were pretreated with 5 μg of α-defensin-1/ml or CAF (50%K) for 16 h. Cells were washed and placed in fresh media without (wash off) or with (add back) α-defensin-1 or CAF prior to infection with HIV-1vsv. Luciferase activity (in RLU) was measured at 48 h p.i. The results (means ± standard deviations) from triplicate determinations in a single experiment are presented as histograms.
