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α-Defensin-1 does not induce STAT1 activation. HeLa-CD4 cells were treated for 15 min with 1 μg of α-defensin-1/ml (lane 2), 10% CAF (lane 3), or 10 ng of IFN-γ/ml (lane 4). Untreated control is shown in lane 1. WCE were prepared and analyzed by Western blot analysis with an antibody against phospho-STAT1 antibody. The blot was then stripped and reprobed with STAT1 antibody.
