FIG. 2.

Pairs of micrographs of 143-tk⁻ and Vero cells transfected with pwtgK-MTS and double stained with antibody to Myc (A, C, and E), to calnexin (B and D), or ConA (F). Anti-Myc antibody only stained the transfected cells, whereas the antibody to calnexin and the lectin stained all cells. In panels C and D, transfected cells were superinfected with R7032 at 24 h after transfection and fixed 10 h later. Panels A, C, and E show that anti-Myc staining was reticular and diffused to the cytoplasm, with a pattern indistinguishable from that of calnexin or ConA (arrows), in both uninfected and R7032-infected cells. Panels A to D, 143-tk⁻ cells; magnification, ×40. Panels E and F, Vero cells; magnification, ×63.