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HSV-1-specific IgA in mucosal washings collected from the eye (EW) and vagina (VW) of HSV-1-vaccinated and mock-vaccinated mice as determined by ELISA. Endpoint titers were calculated by regression analysis for individual mice (n = 20), and mean values ± standard errors were determined. An asterisk shows significant difference between HSV-1-vaccinated and mock-vaccinated mice as judged by Student's t test (P < 0.05).
