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Effect of time of compound-1453 addition on BVDV NS3 protein expression. MDBK cells were mock infected (A) or infected with wt (B, E, F, G, H, and I) or 1453r (C and D) virus at an MOI of 3. Compound-1453 was added at 0 (D and E), 2 (F), 5 (G), 7 (H), and 9 (I) h p.i., and using anti-NS3 MAb 20.10.6, cells were processed for indirect immunofluorescence at 23 h p.i.
