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Effect of compound-1453 on the membrane-based BVDV wt (A) and 1453r (B) replicase activities. The same amount of protein (5 μg) from each membrane preparation was used for the assay. Each concentration of compound-1453 was run in duplicate as indicated above the autoradiogram. RNA products from these reactions were analyzed by electrophoresis on a 1% agarose gel. Sizes of RNA markers are indicated on the right.
