FIG. 8.

HCMV infection prevents TNF-α-mediated induction of JNK. Control U373 cells (lanes 1 to 4) or U373 cells infected overnight with AD169 (5 PFU/cell) (lanes 5 to 8) were left untreated (lanes 1, 3, 5, and 7) or treated with TNF-α (lanes 2, 4, 6, and 8). Cell extracts were then assayed for JNK activity using GST (lanes 1, 2, 5, and 6) or GST-Jun (lanes 3, 4, 7, and 8) as targets for in vitro phosphorylation with [³²P]ATP. Phosphorylation of GST or GST-Jun was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography. The positions of molecular mass markers (in kilodaltons) are shown to the right of the gel.