FIG. 5.

GFP transduction ability of mutants in HeLa C12 cells. Cells were infected with wild-type rAAV or mutant virus at an MOI of 500 genomic particles and an Ad5 MOI of 10 PFU per cell. Twenty-four hours postinfection, cells were fixed with 2% paraformaldehyde and the number of GFP-positive cells was determined by FACS analysis.