FIG. 6.

Binding and uptake of rAAV2 and R585A/R588A in HeLa C12 cells. (A) 10⁶ cells were infected with rAAV2 or R585/R588A at an MOI of 1,000 genome-containing particles per cell. At the indicated times, infection medium was removed and saved. The cells were washed and harvested, and Hirt DNA was extracted from both the infection medium and the cell pellet. Southern analysis was performed using an [α-³²P]dATP-labeled GFP probe. (B) The percentage of bound and internalized DNA was calculated by dividing the total DNA present in both the medium and the cell pellet by the amount bound and internalized for each time point. The average of three determinations is shown. Error bars indicate a standard deviation.