TABLE 1.

Substitutions found in FMDVs H₉₅⁵, REDpt60, and MARLS subjected to treatments that did not lead to viral extinction

Viral population and treatmenta	Substitutions in 2Cb	Substitutions in the capsidb:
		VP3	VP1
H955
G (4 mM)p5A	M159→L	ND	ND
G (4 mM)p5B	M159→L	ND	ND
H (1 mg/ml)p5A	ND	K173→T	—
H (1 mg/ml)p5B	ND	K173→T	—
G (2 mM) Hp5A	—	K173→E	—
G (2 mM) Hp5B	—	K173→K/E	—
REDpt60
G (4 mM)p5A	M159→M/L	ND	ND
G (4 mM)p5B	M159→L	ND	ND
H (1 mg/ml)p5A	ND	—	R133→Q
H (1 mg/ml)p5B	ND	—	R133→Q
MARLS
G (4 mM)p5A	M159→M/L	ND	ND
G (4 mM)p5B	C141→C/S	ND	ND
H (1 mg/ml)p5A	ND	T67→T/I, D68→D/H	K206→K/I, D52→D/A
H (1 mg/ml)p5B	ND	D68→D/H	—
G (4 mM) H (1 mg/ml)p5A	K169→R	T67→T/I, D68→D/H	N60→T
G (4 mM) H (1 mg/ml)p5B	C141→S	K173→E	S144→L
FU (200 μg/ml) G (4 mM)p5A	M159→L	ND	ND
FU (200 μg/ml) G (4 mM)p5B	M159→L	ND	ND
FU (200 μg/ml) H (1 mg/ml)p5B	ND	K218→K/E	K206→Q
G (8 mM) H (1 mg/ml)p5A	K169→R	—	—
G (8 mM) H (1 mg/ml)p5B	M159→L	M128→T	—

^aThe viral populations analyzed and the antiviral treatments used are described in the text. The viral populations analyzed are those described in Fig. 3; p indicates the passage number, and A and B distinguish duplicate series.

^bThe single-letter amino acid code is used. Amino acids are numbered independently for each protein (16, 17). When the antiviral treatment used contained G, the 2C protein was sequenced; when H was used, the capsid-coding region of the virus was sequenced; when both G and H were used, both regions of the genome were sequenced. Mixtures are indicated with the two amino acids separated with a slash. Substitutions are determined in each case by comparison with the consensus sequence of the corresponding parental virus. No amino acid substitutions were found in VP4 and VP2. —, absence of substitution; ND, not determined.