FIG. 3.
DNA binding and CKII phosphorylation of CarDSa. (A) Binding of CarDSa (0.5 μM) to a 169-bp 32P-labeled DNA probe containing the PQRS promoter and the CarD-binding region (∼2 pM; 13,000 cpm) in the presence of 1 μg of poly(dA-dT) (lanes A), 1 μg of poly(dI-dC) (lane I), or 1 μg of poly(dG-dC) (lane G) under the solution conditions indicated in the text. (B) In vitro CKII phosphorylation of CarD, wild-type CarDSa (lane wt) and mutant CarDSa (lane S198A) carried out in the presence of [γ-32P]ATP under the reaction conditions described in Materials and Methods.