TABLE 1.
M. xanthus strains used in this study
| Strain | Phenotypea | Relevant genotypeb | Reference or sourcec |
|---|---|---|---|
| DK1050 | Car+ Fru+ | carD (wild type) | 35 |
| DK1622 | Car+ Fru+ | carD (wild type) | 21 |
| MR1900 | Car− Fru− | carD3 (ΔcarD) | This study (pMR2603 × DK1622) |
| MR1901 | Car+ Fru+ | carD′ | This study (pMR2696 × MR1900) |
| MR1902 | Car+ Fru+ | carDSa | This study (pMR2698 × MR1900) |
| MR1903 | Car+ Fru+ | carDSa(S198A) | This study (pMR2745 × MR1900) |
| MR1904 | Car− Fru− LacZ− Kmr | carD3 carQ::lacZ | pDAH217 × MR1900 |
| MR1905 | Car+ Fru+ LacZi Kmr | carD′ carQ::lacZ | pDAH217 × MR1901 |
| MR1906 | Car+ Fru+ LacZi Kmr | carDSacarQ::lacZ | pDAH217 × MR1902 |
| MR1907 | Car+ Fru+ LacZi Kmr | carDSa(S198A) carQ::lacZ | pDAH217 × MR1903 |
a
Only the phenotypes relevant to this work are indicated. LacZi, light-inducible synthesis of β-galactosidase; LacZ−, low basal levels of β-galactosidase synthesis in the dark or in the light.
b
Only the genotypes relevant to this work are indicated. Relative to that for the wild-type carD gene in DK1622 (or DK1050), an additional 6 bp separates the initiator ATG codon and the ribosomal binding site in strains MR1901, MR1902, and MR1903 and those derived from them (MR1905, MR1906, and MR1907) (see Materials and Methods).
c
All of the strains except the first two were generated by electroporation of the integrative plasmid into the respective strain as indicated here and described in the text.